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 Wysłany: Czw 8:44, 02 Gru 2010 Temat postu: KART CAT Anti-CD20 chimeric antibody Fab 'fragment |
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Anti-CD20 chimeric antibody Fab 'fragment expressed in E. coli
However, Fab 'for the price, and HI for the second price, the Fab' HI perfect competition can not be down. The results show that the Fab 'able to specifically bind CD:. . 97lD ≈ 66lDi. I gourd Da2mDal4kDa (a) (bJ Wai 2SD, ~ PAGE electrophoresis (aj non-reducing conditions: 1 before purification; 2 after purification: (b) reducing conditions: (1) after purification; (2) before purification gL '・ Hek : jhtGated000098.O12.05aI women r% CatedAI 『1o0.O0M111.59M289.01 (b) Figure 3 Competitive inhibition test immune yellow A: negative control: Feb ten HIT3alB: positive control: HI, high-tech communications ten PBSFL1HejghtGaIedO0O044925759 2000 .4 References: 【I) lavJ1,[link widoczny dla zalogowanych],, ClarkEA.BeverleyPC, ela1.JimJ '1, 112n0, 1985.135:3795 [2] StashenkoP, NsdlerLM, HardyRelal 』] 'y) lyJ, l ~ t/1980, 125: 1678:3 jNadlerLM, KarsmeyersIAndersonKC. etalJaintn ~ st1984.74: 332 [4] StashenkoP, NsdlerLMHardyR, el8】. P Ⅻ NatlHighExpressionofChimericCD2oAcad & USA1981.78: 3848 [5] EinleldDA, BrownJP, ValentineMA, etalEl Ⅵ B [V19887: 711:6] Yang Xifeng, sink Cheng,[link widoczny dla zalogowanych], Jin Yuguang and so on. Shanghai Journal of Immunology l990, 10 (2): 65:7] \Fritsch, T. MaNatis, eds Jin Dongyan such as translation, Science Press 1992AntibodyFabFragmentAgainstinEcoliLaiZengzu, XiongDongsheng, XUYuangfu, ZhuZhenpingFanDongmei, PengHui, ShaoXiaofeng,[link widoczny dla zalogowanych], YangChunzheng (TheStateKeyLaboratoryofExperimentalHematology, InstituteofHematology, CAMS & PUMC, Tianjin, 300020) AhstractThegeneofthevariableregionoftheheavychain (VH) andlightchain (VL) wereamplifiedbyPCRmethodfromtheexpressionvectorofanti-humanCD20singlechainFvantibodyfragment (SeFv ),[link widoczny dla zalogowanych], andthentheexpres-sionvector (pYZFcd20) ofchimericantibodyFab'fragmentagainstCD20wasconstructedbycloningVH, VLintotheexpressionvectorpYZF. TheFab'wasexpres ~ dinEcoliTheFab'waspurifiedbyProteinGagarose. ThecompetitiveinhibitionofimmunofluorescenceshowedthattheFab'inhibitedbindingofthemonoclona1antibodyHIdtOCD20onthesurfaceofDaudieel1Keywords: Monoclonalantibody,[link widoczny dla zalogowanych], ChimericantibodyFab'fragment, CD = 0j2 a
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